解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The synthesis of 16 alpha-3H androgens and estrogens is described. 1-(3H)-Acetic acid in the presence of zinc dust reacts with 16 alpha-bromo-17-ketosteroids to produce 16 alpha-3H-17-ketosteroids. This chemical reaction was used to prepare 16 alpha-3H-dehydroepiandrosterone (I) and 16 alpha-3H-estrone acetate (XI) fr...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(81)80016-5
更新日期:1981-02-01 00:00:00
abstract::Synthesis, biochemical and biological testing of the first carborane derivatives of estrogens are described. Estrone 3-carboranylmethyl ether was synthesized in two steps from estrone. Reduction of estrone 3-carboranylmethyl ether with sodium borohydride provided estradiol-17 beta 3-carboranylmethyl ether. Enzyme kine...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(81)80020-7
更新日期:1981-02-01 00:00:00
abstract::Preimplantation rabbit embryos were incubated with pregnenolone and dehydroisoandrosterone under conditions which gave formazan precipitation by the histochemical technique. The metabolic fate of the labeled steroids was assessed simultaneously. There was no concomitant transformation of pregnenolone to progesterone a...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(81)80012-8
更新日期:1981-02-01 00:00:00
abstract::The effect of sodium intake on the aldosterone metabolic clearance rate (MCR) was examined in 5 normal subjects. Measurements were made under conditions where dietary sodium ranged from 10 to 1500 meq/day. There were no consistent changes in MCR over these extremes of sodium intake, and the plasma level of aldosterone...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(81)90002-7
更新日期:1981-01-01 00:00:00
abstract::Radioimmunoassay (RIA) for testosterone (T) in unchromatographed plasma extracts from ATD-treated rats gave spuriously high values for T. Cross-reaction and chromatographic studies subsequently showed that ATD and, to a much greater extent, its metabolite(s) were responsible for this overestimation. Celite column chro...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90054-9
更新日期:1980-12-01 00:00:00
abstract::Mass spectra and fragmentation patterns of the epimeric 17-, 16-, 15- and 14-hydroxy derivatives of 3-methoxy-1,3,5(10)-estratriene are compared. The main fragmentation pathways are differently influenced, depending on the position of the hydroxy group. The different configuration of the hydroxy groups is reflected on...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90077-x
更新日期:1980-11-01 00:00:00
abstract::Corticosterone-and progesterone-binding activity were measured by saturation analysis, with dextran-charcoal separation, in plasma obtained from male and female rats, and a normal male and female human. In plasma from normal male and female rats, progesterone was much less effective than corticosterone in displacing 3...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90009-4
更新日期:1980-09-01 00:00:00
abstract::Radioactive mestranol (ME) and/or ethynylestradiol (EE) were administered to women in Nigeria, Sri Lanka, and the USA, and the types and patterns of radioactive urinary conjugates examined by Sephadex LH-20 chromatography. There are no differences in the total excretion of urinary radioactivity over 3 days. Consistent...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90001-x
更新日期:1980-09-01 00:00:00
abstract::For the purpose of describing the pathway by which estrones are synthesized in the rhesus monkey (Macaca mulatta) corpus luteum (CL), CL were obtained during the midluteal phase of the menstrual cycle and fragments incubated with equimolar amounts of [7-3H]pregnenolone plus [4-14C]progesterone. Metabolites including 3...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90019-7
更新日期:1980-08-01 00:00:00
abstract::A heretofore unknown metabolite of vitamin D3 was isolated from the 1 alpha, 24, 25-trihydroxyvitamin D3 fraction of lipid extracts obtained from plasma of rats which were given intravenous or oral doses of 100 pmol/100 g of either 1 alpha-hydroxyvitamin D3 or 1 alpha, 23-dihydroxyvitamin D3. Doses of 25-250 pmoles of...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90065-3
更新日期:1980-07-01 00:00:00
abstract::Sertoli cells isolated from 17 day old rats were maintained in culture and incubated with [14C]-progesterone for 20 h. The cells and media were extracted with ether/chloroform and the extracts chromatographed two-dimensionally on TLC and the radioactive metabolites visualized by autoradiography. Nine of the metabolite...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(80)80009-2
更新日期:1980-05-01 00:00:00
abstract::A convenient procedure for the synthesis of 5 beta-cholestane-3 alpha,7 alpha,12 alpha,25-tetrol via a modified homologation sequence of the intermediate 3 alpha,7 alpha,12 alpha-triformyloxy-24-oxo-25-diazo-25-homo-5 beta-cholane involving a homogeneous medium is described. This involves treating the intermediate alp...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90144-0
更新日期:1980-04-01 00:00:00
abstract::Two new sterols, (24E)-24-n-propylidenecholesterol and 24 epsilon-n-propylcholesterol, were isolated from a cultured marine Chrysophyte. Since most of the steroids found in marine invertebrates are unchanged or modified sterols of algal or symbiotic origin, the discovery of these two unusual sterols in a unicellular a...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90104-x
更新日期:1980-02-01 00:00:00
abstract::7 alpha- and 7 beta-carboxymethyl-derivatives of 17-hydroxyprogesterone and 11-deoxycortisol have been synthesized. After coupling to bovine serum albumin, they were used to elicit antibodies in rabbits. No major difference in the steroid specificity of the antisera was observed when either 7 alpha- or 7 beta-epimers ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90112-9
更新日期:1980-01-01 00:00:00
abstract::Seventeen cholesteryl alkyl ethers were synthesized through alcoholysis of cholesterol p-toluenesulfonate. This method was found superior to the etherification of sodium or potassium cholesterylate with alkyl halides or methanesulfonates, especially for the preparation of long-chain unsaturated aklyl ethers of [7(m)-3...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90109-9
更新日期:1980-01-01 00:00:00
abstract::In an attempt to justify use of trypsin to achieve more thorough dispersion of luteal cell clumps in vitro, progesterone (P) production by collagenase dispersed monkey luteal cells from the mid-luteal phase corpus luteum (CL) was examined in vitro either after 10 min, or continuous (3h) exposure to trypsin (TR). In th...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90110-5
更新日期:1980-01-01 00:00:00
abstract::The apolar ecdysteroid present in the developing embryo of the blue crab, Callinectes sapidus Rathbun, is tentatively identified as ponasterone A (2 beta, 14 alpha, 20,22-pentahydroxy-5, beta-cholest-7-en-6-one) on the basis of chromatographic, immunological, and mass spectral evidence. The apolar ecdysteroid present ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90092-8
更新日期:1979-12-01 00:00:00
abstract::Circulation levels of estrone sulfate (E1S) and dehydroepiandrosterone sulfate (DHAS) have been measured in plasma using a radioimmunoassay for estrone and dehydroepiandrosterone following extraction and hydrolysis of the sulfate. The mean +/- SE concentrations of E1S and DHAS in normal men were 458 +/- 25 pg/ml and 1...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(79)80017-3
更新日期:1979-11-01 00:00:00
abstract::As demonstrated for pregnenolone, saturated ketones are conveniently alpha-hydroxymethylated by their transformation into a lithium enolate and by the reaction of the latter with formaldehydr. The 17-hydroxymethylpregnenolone prepared by this method in very good yield was readily converted to 17-hydroxymethylprogester...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(79)80021-5
更新日期:1979-11-01 00:00:00
abstract::Six naturally occurring C27 ecdysteroids were isolated and identified from the tobacco hornworm during pupal-adult development five days after peak titer of molting hormone activity. In order of decreasing quantities the hormones were: 20,26-dihydroxyecdysone, 3-epi-20-hydroxyecdysone, 20hydroxyecdysone, 3-epi-20,26-d...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90084-9
更新日期:1979-09-01 00:00:00
abstract::The sterol mixture of the southern Japan's soft coral, Sarcophyton glaucum, was found to contain 11 sterols including a novel sterol, 23,24 xi-dimethylcholesta-5,22-dien-3 beta-ol and a new diunsaturated C29 sterol. 22,23-Dihydrobrassicasterol and gorgosterol were the major components in free- and esterified sterols r...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90079-5
更新日期:1979-09-01 00:00:00
abstract::Bile acid derivatives, with and without C-3 sulfate groups, and having either the diazo- or halomethylketone moieties, have been synthesized in good yield and purity. The synthetic sequence, COOH leads to COC1 leads to COCHN2 leads to COCH2X, was used with deoxycholic and cholic acids, which requires carefully control...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90046-1
更新日期:1979-08-01 00:00:00
abstract::The mass spectral elimination of water in epimeric 1,3-diols of vitamin D3 (colecalciferol) series has been investigated. It was found that the mass spectra of these steroisomers differ sharply in the relative intensities of the ions M-H2O (m/e 382) and a-H2O (m/e +/- 34), where ion a (formed via formal cleavage of th...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90039-4
更新日期:1979-05-01 00:00:00
abstract::In addition to using radioimmunoassays for the determination of estrogens and other steroids, the possibility of using mass fragmentography for analysis was investigated. t-Butyldimethylsilyl chloride was selected as a reagent for derivatisation because it forms rather stable silylethers. In all the mass-spectra obrai...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90013-8
更新日期:1979-04-01 00:00:00
abstract::A sensitive accurate assay for the placental microsomal 3 beta-hydroxysteroid dehydrogenase (E.C.1.1.1.51) has been developed using tritiated substrates. Kinetic analysis of the enzyme with 3 beta-hydroxy-5-androsten-17-one and 3 beta-hydroxy-5-pregnen-20-one indicates that the apparent Km values for these substrates ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90018-7
更新日期:1979-04-01 00:00:00
abstract::Optimal assay conditions for cholesterol 7 alpha-hydroxylase activity in swine liver microsomes were determined. The enzyme activity is induced three-fold by feeding cholestyramine to the swine. This suggests that cholesterol 7 alpha-hydroxylase is likely to be the rate-limiting enzyme for biosynthesis of bile acids i...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90008-4
更新日期:1979-03-01 00:00:00
abstract::2-Phenyl-6-methyl-4-oxo-4,5,6,7-tetrahydrobenzofuran (II) is converted into racemic 2-phenyl-7-methyl-3-oxa-A-nor-14 beta-estra-1,5(10),6,8-tetraen-17 alpha-ol (VIII). ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90023-0
更新日期:1979-02-01 00:00:00
abstract::A technique has been developed in which progesterone may be measured by radioimmunoassay in unextracted serum. The method depends on the displacement of progesterone from serum binding proteins by danazol, [17 alpha-pregn-4-en-20-yno (2,3-d) isoxazol-17-ol], a compound which also blocks recombination of free progester...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90021-7
更新日期:1979-02-01 00:00:00
abstract::The properties of 5-ene-3 beta hydroxysteroid oxidoreductase (3 beta-HSD) from human placental homogenates were studied in vitro. The apparent Michaelis constants for 3 beta-HSD with the substrates pregnenolone (delta 5P) and dehydroepiandrosterone (DHA) were 170 nM and nM respectively. The optimal pH for both these s...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(79)80002-1
更新日期:1979-01-01 00:00:00
abstract::Steroid delta 4-5 alpha- and delta 4-5 beta-reductase activity was determined in 16 human mammary tumors and 8 DMBA-induced rat mammary tumors using a spectrophotometric assay. Steroid delta 4-5 alpha-reductase was present in all tumors investigated while delta 4-5 beta-reductase was detected in only 6 estrogen recept...
journal_title:Steroids
pub_type: 历史文章,杂志文章
doi:10.1016/s0039-128x(79)80010-0
更新日期:1979-01-01 00:00:00
abstract::17 beta-Hydroxysteroid dehydrogenase (HSD) was studied in endometrium removed surgically from intact female monkeys in the luteal phase of the menstrual cycle. Also castrated monkeys (N=20) implanted with estradiol (E2) and progesterone (P) in silastic capsules were used to investigate the hormonal dependence of this ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90139-9
更新日期:1979-01-01 00:00:00
abstract::A procedure utilizing co-chromatography and complementary antiserum comparisons was employed to assess the specificity of a cortisol radioimmunoassay for use in the chronically catheterized fetal sheep preparation. Complementary antiserum comparisons is a technique by which two different cortisol antisera, prepared fr...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(79)80005-7
更新日期:1979-01-01 00:00:00
abstract::[7beta-3H]-(24R and 24S)-27-nor-24-methyl-3alpha,7alpha-dihydroxy-5beta-cholestan-26-oic acids and [7beta-3H]-27-nor-3alpha,7alpha-dihydroxy-5beta-cholestan-26-oic acid (C27 and C26 bile acids having the same nuclear configuration as cheno-deoxycholic acid and its precursor, 3alpha,7alpha-dihydroxy-5beta-cholestan-26-...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90076-4
更新日期:1978-12-01 00:00:00
abstract::Addition of soluble supernatant to testis microsomes results in 42% increase in steroid 17,20-lyase activity and a 65% increase in 17alpha-hydroxylase activity. This stimulatory activity could be partially purified by salt fractionation. The activating factor(s) was not removed by dialysis nor did it appear to be lipi...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90090-9
更新日期:1978-10-01 00:00:00
abstract::Trilostane is a competitive inhibitor of 3beta-hydroxysteroid dehydrogenase. In vitro, the drug inhibits conversion of pregnenolone to progesterone but does not alter conversion of cholesterol to pregnenolone nor progesterone to corticoid hormones. When given orally to rats, trilostane inhibits corticosterone and aldo...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90010-7
更新日期:1978-09-01 00:00:00
abstract::Cortisol 21-amine (21-amino-11beta,17-dihydroxy-4-pregnene-3,20-dione) was prepared and an enzyme immunoassay for cortisol in serum was established using cortisol 21-amine conjugated with alkaline phosphatase. The minimal amount of cortisol detected was 1ng/tube and the measurable range was from 1 to 80 microgram/d1, ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90106-x
更新日期:1978-07-01 00:00:00
abstract::By the conventional methods of gas liquid chromatography (GLC) as well as mass spectrometry, 3beta,7alpha-dihydroxychol-5-en-24-oic acid (delta5-acid), a key intermediate of chenodeoxycholic acid biogenesis and its metabolic by-product, 3alpha,7alpha-dihydroxychol-4-en-24-oic acid (delta4-acid) have not yet been ident...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90102-2
更新日期:1978-07-01 00:00:00
abstract::The free sterol mixture of the sponge Psammaplysilla purpurea was shown to contain aplysterol as the major constituent. In addition to other sterols such as 5,7-cholestadien-3beta-ol, cholesterol, 5alpha-cholestan-3beta-ol, 24epsilon-methylcholesta-5,22-dien-3beta-ol, 24epsilon--methylcholesterol, 24epsilon-ethylchole...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(78)80046-4
更新日期:1978-06-01 00:00:00
abstract::A radio-gas chromatographic method has been devised for the estimation of 11-oxotestosterone (17beta-hydroxyandrost-4-ene-3, 11-dione) in fish plasma samples which provides an independent means of validating the radioimmunoassay described earlier. Estimates of the concentration of 11-oxotestosterone in a sample of mal...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(78)80009-9
更新日期:1978-05-01 00:00:00
abstract::15alpha-Hydroxydehydroisoandrosterone and 15alpha-hydroxypregnenolone were isolated from hydrolyzed extracts of human late pregnancy urine and identified by means of the isotope dilution technique. In two separate determinations the excretion rate of 15alpha-hydroxydehydroisoandrosterone was found to be 1.7 and 3.2 mi...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(78)80002-6
更新日期:1978-05-01 00:00:00